The work programme in DINAMIC is based on: (i) state-of-the-art prospective and genetically well-characterized human cohorts to gain detailed insight into associations between dietary habits, the gut microbiome and cardiometabolic health parameters; and (ii) targeted nutritional and microbiota interventions to study causal relationships between specific diet-responsive microbial taxa and related metabolites and the development of metabolic syndrome as risk factor for cardiovascular diseases. The work is underpinned by established pipelines for assessment of 16S rRNA gene diversity and composition, as well as state-of-the-art shotgun metagenomics, metabolomics and data integration platforms. Hence, the complementary use of large-scale and targeted strategies, explorative and interventional approaches, as well as standardization innovative assessment of the gut microbiota within the DINAMIC consortium will deliver penetrating insights into nutritional strategies to develop and validate practices for modulation of the intestinal microbiome to promote metabolic and cardiovascular health.
In work package 1, we analyse faecal samples at the level of microbiota structure (16S inventory) and functions (meta-genomes and -metabolomes) in relation to host phenotype and genotype in the context of two large-scale human cohorts: KORA and TwinsUK.
In work package 2, we perform dietary interventions with an isocaloric Mediterranean diet (Med-D) coupled with detailed host phenotyping and gut microbiota profiling during and after intervention to assess causal effects of the interplay between diet and the gut microbiome in populations at high risk for CMD.
In work package 3, we share and integrate data to perform meta-analyses and standardise methods to analyse and understand human diet-gut microbiome interactions. Partners in the areas of metagenomics and metabolomics represent the technical foundation of the consortium and guarantee uniformity and high quality of analysis.